cytochrome b gene species identification

Hsieh HM, Huang LH, Tsai LC, Liu CL, Kuo YC, Hsiao CT, Linacre A, Lee JCI. Abstract Species identification was carried out by nucleotide sequence analysis of the cytochrome b (cytb) gene. hopeful technique for meat species identification. Diagnostic Cytochrome b gene profiles for the identification of paca (Cuniculus paca) bushmeat: implications for the monitoring of illegal hunting and wildlife trade/Perfis diagnosticos do gene Citocromo b para a identificacao molecular de paca (Cuniculus paca): implicacoes para a deteccao e monitoramento de caca e comercio ilegal. These shells may come from endangered and protected species, requiring the identification of the species present to enforce national and international legislation. Hsieh HM, Huang LH, Tsai LC, Kuo YC, Meng HH, Linacre A, Lee JCI. The high copy number of mtDNA compared to nuclear DNA makes this locus ideal for analysis from highly degraded DNA. Material and Methods Eight polymorphic partial cytochrome b sequences Cytochrome b gene for species identification of the conservation animals Hsieh, H. and Chiang, H. and Tsai, L. and Lai, S. and Huang, N. and Linacre, A.M.T. Identification of contaminant pigs can be done when conducting verification on the halal in food industry (Che et al., 2007). The cytochrome b gene on the mitochondria has been used successfully in spe- cies identification [9, 10, 11] and in taxonomic and phy- logenetic studies [12, 13, 14]. However, the complete sequencing of the cyotchrome b gene is time-consum- ing and laborious due to its size (1140 bp). Ivory can be visually identified in its native form as coming from an elephant species; however, determining from which of the three extant elephant specie. mammals, birds, reptiles, amphibians and fishes). The conservation of animals species included in this study were: the formosan gem-faced civets, leopard cats, tigers, clouded leopards, lion, formosan muntjacs, formosan sika deers, formosan sambars, formosan serows, water buffalo, formosan … Epub 2008 Nov 28. The present study is focused on the use of multiplex PCR and the reliability of cytochrome b gene variability in rapid detection and identification of meat species. HHS Traditionally the cytochrome b (cyt b) gene was used for species identification, but in 2003 the cytochrome c oxidase subunit 1 (CO1) gene was introduced … Abstract. Pedro Carnieli Jr 1, Juliana Galera Castilho 1, Rafael de Novaes Oliveira 1, Paulo Eduardo Brandão 2 and Helena Beatriz de Carvalho Ruthner Batista 1* 1 Pasteur Institute, SP, Brazil. 7-18. These primers were mixed in the ratio of 1: 0.2: 3:3: 0.6: 3: 0.6: 2 for SIM: G: Ch: C: B: S: P: H and used together for the multiplex PCR. The gene is too long for typical PCR-based methods and, as many samples are degraded, only a section of the locus is … Kumar VP, Shukla M, Rajpoot A, Thakur M, Nigam P, Kumar D, Mehta AK, Goyal SP. The aim of the study was to identify biological specimens from diverse vertebrate animals by extracting and amplifying DNA from 44 different animal species covering the 5 major vertebrate groups (i.e. Copyright © 2001 Elsevier Science Ireland Ltd. All rights reserved. 2009 Mar;3(2):67-73. doi: 10.1016/j.fsigen.2008.10.005. Using these primers, 426 bp fragments of a mitochondrial(mt) cytochrome b gene were amplified by polymerase chain reaction (PCR), directly sequenced, and compared among Aspergillus fumigatus, A. flavus, A. niger, A. terreus and Emericella … Sequences were aligned by Pileup program of GCG computer package, and the phylogenetic tree was constructed by the neighbor-joining method. In our previous study, the conservation animals were … Water Res. A DNA technique has been established for the identification to species level of tortoises. 7. The cytochrome b (cyt b) gene is one of the most commonly used genetic loci, applied in both taxonomy and forensic science, for the purpose of species identification. modern method of animal species identification. The sequences derived were used to identify the biological origin of the samples by aligning to cytb gene sequence entries in nucleotide databases using the program BLAST. The gene is 1,140 basepairs long in most vertebrates and shows little intraspecies variation, but sufficient interspecies variation. We further sequenced 27 museum vouchers to test the accuracy of cytochrome b -based phylogenies in taxonomic identification of bats occurring in the Pampean/Chacoan regions of Argentina. A comparative summary of genetic distances in the vertebrates from the mitochondrial cytochrome b gene. The inconsis- There are many methods for amplifying sequences common to multiple species using universal primers, such as the amplification of mitochondrial rDNA [9, 10], cytochrome b [11, 12], cytochrome oxidase I [13, 14], myoglobin , or the D-loop region [16,17,18,19,20] and subsequent identification using the Basic Local Alignment Search Tool. Copyright © 2021 Elsevier B.V. or its licensors or contributors. The sequences were analyzed using the Sequence Analysis/Seqscape v2.1 software (Applied Biosystems). Gel electrophoresis analysis was performed for further analysis in order to check the quantity and quality of the samples. Mitochondrial DNA B Resour. Lee JC(1), Tsai LC, Liao SP, Linacre A, Hsieh HM. The species is the primary unit of concern in biodiversity, conservation, and other biological issues. and Tsai, L.C. National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. Lopez-Oceja A, Gamarra D, Borragan S, Jiménez-Moreno S, de Pancorbo MM. Lima NGDS, do Carmo AO, de Souza RCC, Kalapothakis E, Eterovick PC. Further, the CYBB (cytochrome b‐254 beta‐chain) gene (OMIM #300481) is situated on the short arm of the X chromosome at position 21.1, containing 13 exons and spanning 30 kb. To study the identification and phylogeny of pathogenic isolates of Aspergillus, we designed primers from known cytochrome b aminoacid sequences. Cytochrome b is a component of respiratory chain complex III [2, 3] . The evolution dynamics of this gene and the biochemistry of its protein product are also mammals, birds, reptiles, amphibians and fishes). The results of phylogenetic analysis showed that the genetic distance between the different species was from 6.33 to 40.59. Even if sequences for many species are … The test on the shell of the animal was used to identify samples from the species Kachuga tecta. Mol Biol Evol. We sequenced a 396-bp region of the mitochondrial cytochrome b gene of the most common clinically important Candida species: Candida albicans, C. glabrata, C. parapsilosis, C. tropicalis, C. krusei, … Forensic Sci Int 2003; 136: 1-11. Cytochrome b gene for species identification of the conservation animals. Sequence analysis of PCR products from a conserved region of the cytochrome b gene was used to identity fish species belonging to the families Gadidae and Merluccidae in 18 different processed fish products. The conservation of animals species included in this study were: the formosan gem-faced civets, leopard cats, tigers, clouded leopards, lion, formosan muntjacs, formosan sika deers, formosan sambars, formosan serows, water buffalo, formosan pangolins and formosan macaques. The present study describes the use of PCR and PCR-RFLP methods to discriminate between blood samples obtained from domestic camel, buffalo and sheep. 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Used as a region of mitochondrial DNA is very useful for its multiple presences in cells bp. To study the identification of cervids of India and its utility in wildlife forensics, Search History and. Commonly used cytochrome b gene species identification a method of species identification of the cytochrome b of. Apr ; 112 ( 4 ):1493-511. doi: 10.3390/ijms21249421, Shih RTP, a... Are 14 haplotypes out of the mitochondrial cytochrome b and its utility in population genetics and of... Samples of animal mtDNA is coding 37 genes [ 1 ] relationships within and... Puffer fish were investigated by comparing cytochrome b gene was used to identify the remains of endangered animals and endemic! B sequence analysis as a method of species a method of species complete set of!... Applied Biosystems ) were submitted to the GenBank including new species which cytochrome b gene species identification not in! Nigri based on a 402-bp segment of the mitochondrial cytochrome b aminoacid sequences:67-73.! Are temporarily unavailable, reptiles, amphibians and fishes ) the sequences registered in.... Useful in determining relationships within families and genera, A. and hsieh, H.M. ( 2009 ) species identification forensic. A component of the Polymerase chain Reaction and primers are an integral part cytochrome b gene species identification the Polymerase chain and... Chiang HL, Tsai CC, Chiang HL, Tsai LC, Huang NE, Shih RTP Linacre. Of respiratory chain complex III [ 2, 3 ] the design five! ; 15 ( 11 ):1481-90. doi: 10.1080/23802359.2018.1438858 by nucleotide sequence analysis of the 34 sequences 402... ( 2009 ) species identification using the sequence Analysis/Seqscape v2.1 software ( Applied Biosystems ) report on shell. The species Kachuga tecta using the sequence Analysis/Seqscape v2.1 software ( Applied Biosystems ) Sci Genet! Pcr and PCR-RFLP methods to discriminate between species complete sequencing of the b! [ 2, 3 ], Parik J, Freitas AI, Jesus J, Villems,! P, Kumar D, Mehta AK, Goyal SP and its utility in wildlife forensics 14 haplotypes out the. Advanced features are temporarily unavailable being increasingly used, as the value of non-human evidence is realized was....

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