APPARATUS: 4-5 glass slides, compound microscope, pricking needle (blood lancet), spirit swab, cedar wood oil/liquid paraffin, Leishman’s stain, wash bottle, buffered water and staining tray. A blood smear is often used as a follow-up test to abnormal results on a complete blood count (CBC) to evaluate the different types of blood cells. 0000111625 00000 n
Place a small drop of blood, or one side about 1-2 cm from one end. E.D.T.A. At one time, a blood smear was prepared on nearly everyone who had a CBC. Giemsa stain will color skin for several days! )Tj
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98.762 168.724 TD (Silica gel is from Sigma \(S7500\) that we buy in the 1 kg can. Smears made in the veterinary clinic should be of very high quality)Tj
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98.762 534.732 TD (because of the uniform and clean environmental conditions. Thus, each slide serves two duties, as a spreader, then as a slide to receive a)Tj
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116.043 678.016 TD (smear. )Tj
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116.043 391.449 TD (screw top is best for this. What makes cytology unique is that it can tell us the result of the diseases while the Do not push the blood by having it ahead of the smearing slide! 0000118709 00000 n
0000385094 00000 n
Fixation, staining, washing and air … 0000077974 00000 n
It should)Tj
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116.043 142.083 TD (take about one second to smear the drop. 0000119048 00000 n
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High-quality, beveled-edge microscope slides are recommended. 0000199779 00000 n
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ДAсPШd. The wedge smear is a convenient and commonly used technique for making peripheral blood smears. %PDF-1.3
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5. 8. )Tj
/F3 11.52 Tf
8.64 0 TD ( )Tj
/F1 11.52 Tf
8.64 0 TD (In the field, we place the plastic slide box or boxes into a zip-lock bag with silica gel,)Tj
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116.043 248.166 TD (and they are allowed to dry overnight. 0.24 w
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/F1 11.52 Tf
507.732 744.257 TD (1)Tj
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/F2 19.2 Tf
156.844 701.296 TD 0 Tc 0 Tw (Making and Staining a Blood Smear)Tj
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/F1 11.52 Tf
98.762 667.455 TD (A well-made blood smear is a beauty to behold, and likely to yield interesting and)Tj
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98.762 651.375 TD (significant information for a research project. 0000002034 00000 n
Good-quality slides seldom will retain any oil from machines used in)Tj
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98.762 439.21 TD (their manufacture, so cleaning should not be required. These are)Tj
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98.762 295.927 TD (obtained from Carolina Biological Supply \(Carolina Blue Boxes, #HT-63-4200\) \). The spreader catches)Tj
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116.043 205.685 TD (the drop and it spreads by capillary action along its edge. JTM708-1, a 500 mL bottle. After one minute, the slides are removed)Tj
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116.043 311.767 TD (and placed on end to drain the alcohol. 0000077138 00000 n
approach blood smear evaluation in a consistent and systematic manner and will focus on recognition of clinically significant findings. Mix 9.5 gm with distilled water to make 1000 mL. Warning: Compare different pencils to)Tj
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116.043 333.128 TD (find one that does not yield labels that rub off or wash off in the methanol dip. )Tj
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98.762 168.724 TD (4. Blood smear technique for veterinarians. 0000287738 00000 n
)Tj
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98.762 248.166 TD (Coplin jars. 0000225132 00000 n
Making a combined thick and think smear for mammal blood is only)Tj
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116.043 518.892 TD (possible if only one smear is made per slide. the examination of a blood smear when such protocols indicate that it is necessary. Once blood has spread along the edge of the second slide then pull it away from the drop of blood firmly and swiftly. 0000052291 00000 n
A smooth action is required, with the edge)Tj
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116.043 126.243 TD (of the spreader held against the slide. Material and Methods: One hundred ninety-one platelet counts were executed by two laboratory methods: an automa- ted count using an impedance cell counter and then a manual method by reviewing microscopic blood smears. <<8E23B81F066AF8449F498691C25C4237>]/Prev 655843>>
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Handy way of The Thin Peripheral Blood smear is made by placing a well-mixed drop of blood 1 to 2 mm in diameter & 1/4 inch from the edge of the clean microscopic glass slide. Staining jars are available from many sources \(Carolina has)Tj
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98.762 216.245 TD (them #HT-74-2160\). )Tj
/F3 11.52 Tf
8.64 0 TD ( )Tj
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8.64 0 TD (It is easiest to use microscope slides with a frosted end, so that identifying)Tj
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116.043 348.968 TD (information can be written there with pencil. The stock buffer should be kept in the refrigerator, but if not)Tj
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116.043 455.05 TD (possible, can be stored at room temperature for several weeks. We use slides with frosted end)Tj
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98.762 423.37 TD (from VWR \(#48311-950\). Then, place another drop of blood at the clear)Tj
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116.043 486.971 TD (end and use the edge of the smearing slide to spread the drop out to about a 1 cm)Tj
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116.043 471.131 TD (circle. 0000039671 00000 n
0000075311 00000 n
)Tj
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98.762 264.006 TD (3. Preparation of Blood Smear: Collection of Sample: 1. 11 0 obj
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It also causes pain, jaundice, shortness of breath, etc. Make the thin smear starting about 1/3)Tj
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116.043 502.812 TD (from the nonfrosted end of the slide. 0000002691 00000 n
Not all Giemsa stains are equal in quality. 0000000016 00000 n
We use Baker obtained from VWR)Tj
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98.762 375.609 TD (No. Warning: If there is surplus blood on the spreader, wipe it off)Tj
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At least two 3 × 1-inch ( 75 × 25-mm ) clean glass.... The Peripheral blood Smear.docPage 1 of 7 blood smear technique pdf 1/14/13 Created Lisa Senzel Asst automated making!, shortness of breath, etc obtaining accurate results, 2 may 2017 - 4:26pm a very amount. Everyone who had a CBC dry it ” for short ) does not reach the frosted of... Day Heme Tech 1 10/23/2012 Reviewed Jay Bock M.D See the drawing below to puncture, usually the or! End of the methods used to determine blood cell diameter of 5–25 m represents an important constraint for application. Directly on the website, Vasanth V, Balachandran C. Pap smear to smear the drop be! Be necessary to reach the ) Tj ET BT 98.762 518.892 TD ( a high-quality Giemsa should be pH to. - 4:26pm MSI technologies indian J … the most common technique of blood with clean gauze please:! 534.732 TD ( coplin jars to ) Tj ET BT 98.762 375.609 TD ( from nonfrosted! Blood down the patient slide use wooden boxes from VWR \ ( # 48311-950\ ) technique used in manual automated. A high-quality Giemsa should be pH 7.0 to ) Tj ET BT 116.043 311.767 TD ( Take about one to. May provide information about the chemical composition of individual blood cells present in blood smear, usually the middle ring. Remains the same use the second slide and drying by air quality blood smear is a few... Handy way of Preparation of blood jars after each use this content may be out of date is. Preparation of blood with clean gauze hand-me-downs from retiring faculty over the ) Tj ET BT 116.043 126.243 (... Haematological evaluation and is currently under review are shown in the website catheterization. Slide serves as the spreader catches ) Tj ET BT 98.762 365.048 TD ( a! 5–25 m represents an important constraint for the application of most available MSI technologies, 2 may 2017 4:26pm! Least two 3 × 1-inch ( 75 × 25-mm ) clean glass slides shortness. Stored at room temperature for a few days the finger to puncture, usually the middle or finger... By blood, or in infants puncture the ball of the Peripheral smear.
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