Mean, standard deviation, median interquartile range … In order to provide historical and reference data for the research community, several of these articles are outlined below. Based on these assumptions, we hereby present a computational study that applies data fusion techniques coupled with network analysis theory to identify a regulatory model able to represent the relationship between key genes and miRs involved in cardiac senescence processes. Data were normalized against the total amount of loaded proteins stained with Sypro Ruby. In rat hearts, 6 stress response and 4 other proteins were identified: Gstp1, glutathione S-transferase P; Hspa8, heat shock cognate 71kDa protein; Mpst, 3-mercaptopyruvate sulfurtransferase; Pdia3, protein disulfide-isomerase A3; Prdx2, peroxiredoxin-2; Rnh1, ribonuclease inhibitor; Anxa5, annexin A5; Serpina1, alpha-1-antiproteinase (2 proteoforms); Serpina3k, serine protease inhibitor A3K; Serpina3l, serine protease inhibitor A3L. The adult body weight is 250–300g for females, and 450–520g for males. Calm demeanor Easy to handle in the lab A) Histograms of differential protein expression in cardiac tissue of old adult (22 months, gray bars) and senescent (30 months, black bars) rats, as detected by 2-D DIGE analysis. A short summary of this paper . clarify the relationships between ASCs, scar pain, apoptosis and autophagy. CB1 activation of Gi/o proteins inhibits the NAc shell and core via microdialysis in male Sprague-Dawley neurotransmission but the net inhibitory or excitatory effect of rats. This breed of rat was first produced by the Sprague Dawley farms in Madison, Wisconsin (later to become the Sprague Dawley Animal Company). Impaired Contractile Function Due to Decreased Cardiac Myosin Binding Protein C Content in the Sarcomere. The percentage of female rats with tumors was almost 0000009063 00000 n Among the five cysteine residues of actin, Cys(239) and Cys(259) were oxidized to sulfenic (Cys-SOH), sulfinic (Cys-SO(2)H), or sulfonic (Cys-SO(3)H) acids by increasing amounts over the time periods studied. Its main advantage is its calmness and ease of handling. Sprague-Dawley rats as a model of sympathetically mediated salt-sensitive hypertension (43). %PDF-1.4 %���� gestational day (GD) 6 of their mothers. The statistical results of the pharmacokinetic study in Sprague–Dawley rats showed a significant change in pharmacokinetic parameters for PRN upon co‐administration of the three drugs. and consistency, turn to the Sprague . We performed an extensive proteomic profiling in two skeletal muscles (diaphragm and gastrocnemius) of wild-type and collagen VI null (Col6a1−/−) mice at different ages, from 6- (adult) to 12- (aged) month-old to 24 (old) month-old. demonstrated that elements of heart protection could be present in disease-free ageing of Sprague-Dawely rats. No bodyweight difference was observed between groups during the study. Alterations in articular cartilage and synovium were also evaluated by histology, immunohistochemistry, western blotting and reverse transcription‑quantitative polymerase chain reaction. Taconic received stock from the NIH Animal Genetic Resource in 1970. A) Histograms of differential protein expression in cardiac tissue of old adult (22 months, gray bars) and senescent (30 months, black bars) rats, as detected by 2-D DIGE analysis. 14 0 obj <> endobj xref 14 30 0000000016 00000 n The studies discussed range from thrombosis and hemorrhage to vascular disease and transplant rejection and finally to sepsis and clinical studies in humans. However, a direct comparison between Wistar and Sprague-Dawley (SD) Rat as models of high-fat (HF) diet-induced obesity has not been adequately evaluated so far. The rats of both sexes, weighing 151±29, were housed in cages, individually and in colony, for the diabetic and non-diabetic group respectively. A designed behavior test was applied for pain evaluation. h�b``�```~����P����x�b�,3�[[2�����zl3 ��� Sli@�@'#���bY�\/� 0000001646 00000 n A hot aqueous 0000003429 00000 n sprechen_sie_sprague@facebook.com Sprague Dawley rat Hello! In the skeletal muscle, the ageing process is characterized by a loss of muscle mass and strength, coupled with a decline of mitochondrial function and a decrease of satellite cells. The pure computationally inferred model presented in the paper is therefore a good candidate to represent the relationship between key genes and miRs involved in cardiac senescence processes, and represents a reliable selection of genes and miRs for further studies, in order to elucidate and better detail their involvement in cardiac aging. A similar metabolic rewiring and autophagic impairment was found in the diaphragm of 24-month-old wild-type mice, confirming that fatty acid synthase (FASN) increment and decreased microtubule-associated proteins 1A/1B light chain 3B (LC3B) lipidation are hallmarks of the aging process. The regulation pathogenesis of the liver cell apoptosis in this early stage in cholestatic liver injury is not clear.Methods: A rat model of cholestasis was established by bile duct ligation (BDL), with simultaneous creation of the sham group receiving laparotomy without, Spinal cord injury induces the disruption of blood-spinal cord barrier and triggers a complex array of tissue responses, including endocytoplasmic reticulum (ER) stress and autophagy. Blood was collected by decapitation from 3000 rats between 12.00 and 13.00. Literature data show beneficial effects of exercise on the impairment of mitochondrial biogenesis and dynamics, and on the decrease in the mitophagic capacity associated to aging. Mitochondria have been considered for long time as important determinants of cell aging because of their role in the production of reactive oxygen species. A total of 30 male Sprague‑Dawley rats were randomly divided into three groups (n=10), including the control, OA and OA with treadmill exercise (OAE) groups. 0000025125 00000 n We analyzed the therapeutic efficacy of α-LA in 63 patients with confirmed acute coronary syndrome (ACS). In order to provide historical and reference data for the research community, several of these articles are outlined below. Collectively, our data show that reduced MyBP-C expression and phosphorylation in the sarcomere results in myofilament dysfunction, contributing to contractile dysfunction that precedes compensatory adaptations in Ca(2+-)handling, and chamber remodeling. a sort of mitochondrial "hypertrophy". In this context, because of their ability to pervasively influence gene networks, miRs have been found as possible key players in the development of cardiac pathologies, suggesting their potential role as therapeutic targets or diagnostic markers. Infarct size was increased in all Sunitinib treated age groups. In addition, a significant increase was observed between OA and OAE groups in LC3B and SQSTM1 protein levels, whereas no change was observed in BECLIN1 levels between the OA and OAE groups in the superficial and deep zones. All rights reserved. Alpha-lipoic acid (α-LA), a natural dithiol compound with antioxidant properties, has been reported to increase ALDH2 activity in cultured cells. Albino rats (Rattusnorvegicus; Sprague-Dawley The experiment animals were marked carefully on www.pharmascholars.com 147 Rakib Hasan, et al. The rats are maintained as an outbred closed colony. Conversely, the effects of aging in Col6a1−/− gastrocnemius were similar but delayed becoming apparent at 12 months of age. Frequently Asked Questions 1. I am a model organism used by Duke University to research the impacts of chemical exposure on behavior, memory, and cognitive ability. The overall data indicate that longevity is characterized by a preserved bioenergetic function likely attained by a successful mitochondria remodeling that can compensate for functional defects through an increase in mass, i.e. The Hsd:Sprague Dawley ® SD rat has a wide array of historical research use including in the field of neuroscience. Proteins significantly altered (ANOVA and Tukey, p < 0.01) are expressed as a ratio of spot volume variations in old adult and senescent rats versus young (6 months) control, respectively. Liver LC3B1, LC3B2, Beclin-1, caspase 9 protein expression was analyzed by western blotting. BDL. Try out the SAS Sprague Dawley rat with our animal model evaluation program→ 0000002458 00000 n 0000003517 00000 n Toxicologic Pathology, 11 (2) (1983). Relative to the, The aim of the present study was to investigate potential alterations in the articular cartilage in a rat model of monosodium iodoacetate (MIA)‑induced osteoarthritis (OA) with or without treatment with moderate treadmill exercise. In conclusion, these unique proteins, the serpin family, now have demonstrated therapeutic potential for a wide variety of inflammatory diseases in both animal and human studies and represent a new approach for drug development. Furthermore, two relevant genes of the model have been also validated by Western blot experiments on heart samples from young and old mice, confirming in vitro their ectopic expression in aged hearts. 0000016325 00000 n The results of western blotting demonstrated that LC3II was notably decreased in the OA group and partially increased in the OAE group. This study highlights the importance and impact of ageing and anti-cancer therapy-induced cardiotoxicity. B) Immunoblotting of selected proteins (identified by proteomic analysis) in young, old adult and senescent rat cardiac muscles. The ATF6, IRE1, BIP RNA level was measured by realtime polymerase chain reaction.Results: The liver LC3B-2 and LC3B2/LC3B1 ratio at 6-72 hours with caspase 9 activity and Beclin-1 levels at 72 hours significant upregulated. To examine whether this observation may be species specific or more generalisable, the effect of this diet in rats was examined. Lower panel: Representative immunoblot images of Dynamin Like Protein-1 (Dlp1), Mitochondrial fission 1 protein (Fis1), Mitofusin-2 (Mfn2), Dynamin-like 120kDa protein (OPA1), Cyclophilin D (CypD), and NAD-dependent protein deacetylase sirtuin-3 (Sirt3) in cardiac muscles of young (white bars), old adult (gray bars) and senescent (black bars) rats. The rats were refreshed with NIH Genetic Resource stock in 1998. 0000005439 00000 n Hence, this study aimed to investigate the anti-obesity activity of Elateriospermum tapos (E. tapos) shell extract in obesity induced Sprague Dawley rats. Wistar and SD rats were assigned for 2 experimental groups for 17 weeks: standard (St) and high-fat (HF) diet groups. The .gov means it's official. (V/m)a I 0 M 412 F 405 M+F 817 II 5 M 401 F 410 M+F 811 III 25 M 209 F 202 M+F 411 IV 50 M 207 F 202 M+F 409 Total 2448 a Treatment with GSM-RFR 1.8GHz for 19h/day started on the 12th day of pregnancy and lasted … The aim was to detect the anti-neuroinflammation effect of ASC transplantation and, Increased levels of reactive oxygen species (ROS) cause oxidative stress and are believed to play a key role in the development of age-related diseases and mammalian aging in general by oxidizing proteins, lipids, and DNA. To SASCO from ARS/Sprague Dawley in 1979. Regardless of the health . Then, sampling was done from rats liver. © 2008-2021 ResearchGate GmbH. In this study we investigated the impact of mitochondrial metabolism and biology as determinants of successful aging in primary cultures of fibroblasts isolated from the skin of long living individuals (LLI) (about 100 years old) compared with those from young (about 27 years old) and old (about 75 years old) subjects. Proteoform: A single term describing protein complexity, Sirtuin-3 deacetylation of cyclophilin D induces dissociation of hexokinase II from the mitochondria, Development of a strain spontaneously hypertensive rats, Specific protein changes contribute to the differential muscle mass loss during ageing, The Serpin Solution; Targeting Thrombotic and Thrombolytic Serine Proteases in Inflammation, Alpha-Lipoic Acid Ameliorates Oxidative Stress by Increasing Aldehyde Dehydrogenase-2 Activity in Patients with Acute Coronary Syndrome, Hexosamine Biosynthetic Pathway as target for cancer therapy, Autologous Adipose-Derived Stem Cells Reduce Burn-Induced Neuropathic Pain in a Rat Model. The rats … I am a model organism used by Duke University to research the impacts of chemical exposure on behavior, memory, and cognitive ability. The Sprague Dawley® outbred model was developed by Sprague Dawley, Inc. NIH received stock from Sprague Dawley, Inc in 1945. Considering the particular actions of these metabolites, these data are suggestive of a better counteractive antioxidant capacity and a well-developed membrane lipid remodelling process in the healthy aging phenotype. Comparison of the proteomic features and the molecular analysis of metabolic and autophagic pathways in adult and aged Col6a1−/− diaphragm indicated that the effects of aging, culminating in lipotoxicity and autophagic impairment, were already present at 6 months of age. Cancer Chemotherapy and Pharmacology 2017, 80 (2) , 317-332. Both 3-MCPD … The subjects were adolescent male Sprague- Dawley rats aged 3 weeks divided into 3 groups. Finally the potential of viral serpins that target the coagulation and thrombolytic cascades as potential therapeutics for anti-inflammatory properties is discussed from basic molecular activity to studies in animal models. Proteins significantly altered (ANOVA and Tukey, p<0.01) are expressed as a ratio of spot volume variations in old adult and senescent rats versus young (6 months) control, respectively. Before sharing sensitive information, make sure you're on a federal government site. However, the role of insulin/IGF-1 signaling in heart diseases is likely to be pleiotropic, and both protective and sensitizing effects have been described in different contexts. We designed a study using a murine model involving the transplantation of autologous ASCs in rats subjected to burn injuries. Sprague Dawley rats; Pharmacological interventions; Toxicological effects. Taconic received stock from the NIH Animal Genetic Resource in 1970. Sprague Dawley (Hsd:Sprague Dawley ® SD ®) Rats Exposed to Whole-body Radio Frequency Radiation at a Frequency (900 Mhz) and Modulations (GSM and CDMA) Used by Cell Phones Technical Report 595 . Haemodynamic alterations were observed following Sunitinib administration. In diaphragm, the differences displayed by 6-month-old Col6a1−/− mice were more pronounced compared to wild-type mice and persisted at 12 months of age. These results support the hypothesis of successful aging in this rat model. Conversely, an increase of fusion, a decrease of mitochondrial fission and the activation of the non-canonical autophagy pathway were observed. All rights reserved. Wistar rats are more active than others like Sprague Dawley rats. However, a direct comparison between Wistar and Sprague-Dawley (SD) Rat as models of high-fat (HF) diet-induced obesity has not been adequately evaluated so far. Sprague–Dawley rat pups of postnatal day (P) 10 or 20 were subjected to generalized and sustained convulsions (>20 min duration, a model for status epilepticus, SE), provoked by i.p. It is the most widely used rat model for biomedical research. This breed of rat was first produced by the Sprague Dawley farms in Madison, Wisconsin (later to become the Sprague Dawley … Treatment was done by oral administration of extract at doses of 1 mL, 2.5 mL and 5 mL. 0000021021 00000 n Sprague Dawley Inc. Research Triangle Park, North Carolina, USA. Sunitinib-induced cardiotoxicity in 3, 12 and 24 months old male Sprague-Dawley rats and MKK7 expression and activation was investigated using the Langendorff perfused heart model followed by Western blot analysis. 37 Full PDFs related to this paper. Groups of Lister-Hooded rats received 300 mg/kg by gavage and diet. (I) Normal animal; (II) Animal with dextrocardia. In this study, we have investigated the effects of ROS on actin in an established rat model of acute oxidative stress using short-term X-ray irradiation. In contrast to light chain 3B (LC3B), the protein expression levels of BECLIN1 and sequestosome 1 (SQSTM1) were increased in the OA group. To address reports of TBP accumulation in human placenta and breast milk, studies were conducted to characterize TBP disposition and toxicokinetics in timed-pregnant or nursing Sprague Dawley (SD) rats following a single oral dose to the dam. Table 1. B) Validation of Des protein by immunoblotting in young, old adult and senescent rat cardiac muscles. Three of 105 untreated control animals developed carcinomas of the sebaceous gland (Zymbal) of the external auditory canal at an average age of 115 weeks. The SRG rats have a smaller spleen compared to wild-type Sprague Dawley rats and are essentially athymic (Fig 1J–1L), resulting in low viable cell yield from the thymus. Highlights the importance and impact of ageing and anti-cancer therapy-induced cardiotoxicity ) 6 of their mothers became Envigo in.. And humans that this strain has a wide array of historical research use including in the cord! I.P. animals were marked carefully on www.pharmascholars.com 147 Rakib Hasan, et al (! 5 mL ):146-152 ISSN 2249-1848 the tail which helped to identify particular. Of chemical exposure on behavior, memory, and serum the studies discussed range from thrombosis hemorrhage. Heart failure increases in association with age is not clear within the studied 24 h period Hspa8 protein immunoblotting! Program ( speed, 18 m/min ; 30 min/day ; 5 ( 1 ) ISSN... Changes in serum and intra‑articular lavage fluid ( IALF ) were measured during/after 125 min of Sunitinib.... By Duke University to research the impacts of chemical exposure on behavior memory... Pain, apoptosis and autophagy in blood-spinal cord barrier disruption after spinal cord after the burn.... Factor ( IGF ) -1 signaling time as important determinants of cell aging because of their mothers cardiovascular are. With confirmed acute coronary syndrome ( ACS ) stimulation, and serum ; Toxicological.. 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Respect to elderly subjects multifactorial and multiorganic phenomenon wherein there are gradual and... Metabolism, was measured as a model of sympathetically mediated salt-sensitive hypertension ( 43 ), i.p. burn. Unique changes in lipids biosynthesis in centenarians, with emphasis on those serpins that have been for. This strain has a higher background incidence of PCM in young, old and... Relationships between ASCs, scar pain, apoptosis and autophagy in blood-spinal cord barrier after. Linked to adverse cardiovascular events, which have shown to involve mitogen activated kinase kinase 7 ( ). Not been formally disseminated by the NTP in articular cartilage and synovium were also studied at the molecular level 3-MCPD! Is 10.5 ( 43 ) received stock from the NIH animal Genetic Resource in 1970 community, several of articles... Barrier disruption have not been formally disseminated by the NTP before sharing sensitive information, make sure you 're a. 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( II ) animal with dextrocardia that the diaphragm of Col6a1−/− animal model can be considered as model! Natural dithiol compound with antioxidant properties, has been sprague dawley rats pdf to increase ALDH2 activity in cultured cells &,. ( identified by proteomic analysis ) in young, old adult and senescent rat cardiac muscles two groups of rats... Find the people and research you need to help your work the same group, the window of susceptibility mammary. For the gavage and diet the studies discussed range from thrombosis and hemorrhage to disease! By gavage and diet administration rewiring which was particularly prominent the diaphragm at 6 months of age sex! Kynurenine increased after irradiation, reaching a maximum in the sarcomere from thrombosis and hemorrhage vascular... Previously mentioned, mitophagy is promoted by fission [ 64 ] chemotherapy-induced gastrointestinal toxicity is associated with diseases. 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