The typical life span is 2.5–3.5 years. Cancer Res. Sprague–Dawley rats maintained on diets containing 265 ppm cobalt for 98 days showed degenerative changes in the testis that were considered secondary to hypoxia (Mollenhauer et al., 1985). In this experiment, ECG with marked ST elevation was used to evaluate successful AMI model. Sprague Dawley rats in health. DOMINIQUE LISON, in Handbook on the Toxicology of Metals (Third Edition), 2007. The typical life span is 2.5–3.5 years. Deficient prepulse inhibition of acoustic startle in Hooded-Wistar rats compared with Sprague-Dawley rats Academic Article Developing a quantitative in vivo tissue reconstitution assay to assess the relative potency of candidate populations of mouse oesophageal epithelial cells Academic Article Immunohistochemical staining was performed on a Ventana Discovery XT automated immunostainer, using Ventana OmniMap anti-rabbit horseradish peroxidase (HRP) secondary antibody incubation followed by diaminobenzidine chromagen. Striatal RTI-55 radioactivities in the caudate nucleus are estimated using Amersham autoradiographic 125I-labeled microscales as the exposure standards. Reproductive organ weights, blood testosterone levels and sperm counts were measured in mature age-matched male rats. Sprague-Dawley rats maintained on diet containing 265 ppm cobalt for 98 days showed degenerative changes in the testes considered to be secondary to hypoxia (Mollenhauer et al., 1985). 0.5, 1 or 3 h afterwards, the animals were decapitated and the brain removed. The Hsd:Sprague Dawley ® SD rat has a wide array of historical research use including in the field of cardiology. The adult body weight is 250–300g for females, and 450–520g for males. 2B). Sperm counts and motility were measured in comparison to various indices of reproductive performance. Female rats were dosed for 14 days prior to mating, and then throughout mating, gestation, and lactation, with an additional week postweaning for 70 days. Sprague Dawley rats in health. F0 male rats were necropsied at termination with sperm count and morphology evaluated in two or three males/group at sacrifice. By having this kind of population, it is possible to conduct experiments on the roles of genes, or conduct experiments that exclude variations in genetics as a factor. They had free access to AltrominR pellet diet except in starvation experiments and to tap water. Organ weights were monitored and histopathological evaluations were made of the pituitary, spleen, liver, kidneys, bone marrow, and reproductive organs. Dawley ® outbred rat model. Durbin PW, Williams MH, Jeung N, Arnold JS. Ethoxycoumarin deethylation was measured by a modification of a method of Ullrich and Weber (11) using a protein concentration of 1 mg/ml and a substrate concentration of 0.5 mM. Its calm temperament and easy handling are welcome features to both scientists and animal lab technicians. Arylhydrocarbon hydroxylase activity was determined by a modification of the method of Gielen et al. The current study aims to investigate the influence of five rare earth elements (REEs) (i.e., lanthanum (La), cerium (Ce), praseodymium (Pr), neodymium (Nd), and gadolinium (Gd)) on the growth of Sprague–Dawley (SD) rats, and to explore the accumulation characteristics of REEs in tissues and organs with different doses as well as the detoxification and elimination of high-dose REEs. Chorioretinal Dysplasia in Sprague-Dawley Rats: Ophthalmologic and Histopathologic Characterization Leah Stein, BS1, Betsy Geddings2, Joshua Bartoe, DVM, MS, DACVO 1,2, Keith Nelson, DVM, PhD, DACVP (1) Michigan State University College of Veterinary Medicine, East Lansing, MI; … However, moderate DR did improve survival by delaying the onset and progression of spontaneous degenerative disease and tumors in Sprague-Dawley rats ( Keenan et al. of [3H]-oestradiol-17β (S.A. 95–100 Ci/mmol); [3H]-dihydrotestosterone (S.A. 44 Ci/mmol) or [3H]-corticosterone (S.A. 55–80 Ci/mmol), dissolved in absolute alcohol—isotonic saline. After a period of at least 40 days, the (adult) conditioned animals were decapitated under volatile agent anesthesia, and the brains were either frozen for the assays described in the later sections, or used for the preparation of hippocampal slices for electrophysiological recordings. The no-observed-effect level (NOEL) and lowest observed effect level (LOEL) were set at 25 and 125 μg kg−1 per day, respectively. It should be noted that image identifications include gender of the animal, but this does not imply there are gender-specific differences in the histologic appearance of the tissue. To SASCO from ARS/Sprague Dawley in 1979. However, during lactation the body weights of pups borne to high-dose parents were reduced compared to controls. Long-Evans rats are thought to come from crosses between Wistar females and a wild Norway rat (Pass and Freeth 1993). Our control animals are injected with an equal volume of 0.9% NaCl with pH adjusted with hydrochloric acid to 2.2, to equal the pH of the ferric chloride solution. In contrast to the Sprague- Dawley rats familiar with Long-Evans rats, Sprague-Dawley rats unfamiliar with Long-Evans rats behaved similarly to control rats (Fig. Rats collected at or before PND 14 were euthanized via intraperitoneal administration of a barbiturate solution. Copyright © 2021 Elsevier B.V. or its licensors or contributors. Gunda Reddy, Marc A. Williams, in Wildlife Toxicity Assessments for Chemicals of Military Concern, 2015. including alloxan- induced diabetic Sprague- Dawley rats. The SD rats from Charles River laboratories (CRL) and Harlan Laboratories (HAR) were monitored for weight gain from the age of 6 weeks to 24 weeks. SD rats produce decreased immobility times in FST following administration of the KOR antagonist nor-binaltorphimine (norBNI) and the novel KOR antagonists 5′-guanidinonaltrindole and 5′-acetamidinoethylnaltrindole (Mague et al., 2003). of evoked potentials in vitro, using either extracellular or intracellular recording techniques. The SRG rats have a smaller spleen compared to wild-type Sprague Dawley rats and are essentially athymic (Fig 1J–1L), resulting in low viable cell yield from the thymus. injections of 20 mg/kg dissolved in peanut oil in 12 h intervals. Testes and epididymides were weighed and examined histopathologically. Staining Characteristics of Lonchocarpus cyanescens Leaf Extract on the Testis of Sprague-Dawley Rats - Volume 18 Issue 4 - Rosemary B. Bassey, Airat A. Bakare, Innocent A. Edagha, Abraham A.A. Osinubi, Ademola A. Oremosu Sprague Dawleys are unable to vomit due to the esophagus entering the stomach. Sprague–Dawley rats, 5–6 rats for each hormone, were injected intravenously under ether anesthesia, 48 h after gonadectomy or andrenalectomy, with 0.5–1.0 µg/100 g body weight (b.w.) Whole brain or hippocampal tissue obtained 40 days post seizure was used to determine ACh innervation density (using choline acetyltransferase immunocytochemistry) and endogenous ACh and GABA content by high-performance liquid chromatography (HPLC) electrospray ionization mass spectrometry, a technique that allows the measurement of small concentrations of endogenous substances (nM) from small volume samples. Gestation for Sprague Dawley on an average is a cycle of 22 days (Sprague Dawley, 2006). Mean, standard deviation, median interquartile range as … By continuing you agree to the use of cookies. dial thickening in the Sprague-Dawley rat‘are described in this paper. RESEARCH DESIGN AND METHODS MG (60 mg/kg/day) or 0.9% saline was administered by continuous infusion with a minipump for 28 days. This study was conducted to investigate absorption characteristics of zinc glycine chelate (Zn–Gly) by evaluating tissues zinc status and the expression of zinc transporters in rats. The incubation procedure and the analysis of T metabolites was performed following the methods described by Kniewald, Massa and Martini[33]. Sprague–Dawley rats, born in the laboratory.were maintained under conditions of controlled temperature and lighting (12 h-12 h) with free access to food and water. In order to examine the degree of variability of an outbred stock obtained from various sources, Sprague Dawley (SD) rat lines obtained from two sources were evaluated for their growth characteristics. Enlarged autoradiographic images are analyzed using the NIH Image software developed by Wayne Rasband (NIMH, NIH) to measure optical densities; gray scales are then converted to rainbow color scales (orange to yellow show high bindings; green to blue depict low bindings). Its main advantage is its calmness and ease of handling. Sprague-Dawley rats of approximately 55 days of age were used. and prepared for stereotaxic infusion of drug (0–10 nmol ferrous citrate and/or 0–16.8 nmol GSNO in 1 μl sterilized Ringer's solution or 0–33.6 nmol peroxynitrite in 1.05 μl 0.3 M NaOH solution) into right or left substantia nigra compacta area (i.n. Specific cell populations of the testis were identified using IHC staining for GATA-4 anti-transcription factor, which is predominantly expressed on Sertoli cell, Leydig cells, and other interstitial cells. Dogs exposed at the high dose showed neurotoxic symptoms such as salivation, hypoactivity, trembling, hyperesthesia, myoclonia, groaning, and prostration before death. Histologic sections of 3–5 µm thickness were collected from each paraffin block, and sections were stained with hematoxylin and eosin (H&E), periodic acid/Schiff-hematoxylin (PAS-H), or luxol fast blue/cresyl violet (LFB/CV). For phenobarbital pretreatment the animals received 3 i.p. A.J. The average litter size of the Sprague Dawley rat is 10.5. For animals collected at PND 0 through 14, the cranial, thoracic, and abdominal cavities were opened at necropsy and the entire carcass was immersion fixed in 10% neutral buffered formalin (NBF). Reproductive organ weights, blood testosterone levels and sperm counts were measured in mature age-matched male rats. Sprague–Dawley rats of both sexes, 10–12 weeks old weighing 125–300 g, were allowed access to commercial rat chow pellets and drinking water ad libitum. 1994a). AMI model is usually judged by electrocardiographic characteristics. The animals had no previous treatment regimen. Surgery on baby rats was performed under hypothermia. Specific cell populations in immune system organs were identified by IHC staining for CD3 (T-cell marker), CD45RA (B-cell marker), and KI67 (proliferation marker). Treatment with 400 ppm cobalt chloride in the diet for 10 weeks induced seminiferous tubule degeneration in mice (Anderson et al., 1992). The Sprague-Dawley strain came from crosses between Wistar females and a "hybrid" male whose origins are unknown. (Index of major rat strains, with histories and strain characteristics). The Sprague Dawley rat is an outbred multipurpose breed of albino rat used extensively in medical research. In order to examine the degree of variability of an outbred stock obtained from various sources, Sprague Dawley (SD) rat lines obtained from two sources were evaluated for their growth characteristics. Male rats were dosed for 14 days prior to mating, and then throughout the mating period for 35 days. Nembutol (50 mg/ml was injected intraperitoneally, 0.1–0.15 ml into each female and 0.15–0.20 ml into each male rat. A left thoracotomy was performed through the fourth intercostal space using a sterile technique. To Charles River in 1996. BPA was administered to pregnant Crl:CD BR Sprague-Dawley rats by gavage at 0, 3.2, 32, or 320 mg/kg/day from gestation day (GD) 11 through postnatal day (PND) 20. Then the SD rats were anesthetized with an intraperitoneal injection of chloral hydrate (350 mg/kg body weight). Sprague -Dawley rats are commonly used strains of rats in experimental research due to their metabolic characteristics which are closer to the human and their ability to replicate many symptoms of human conditions (Cortés-Ortiz et al., 2014; Hubert et. By contrast, outbred populations are used when identical genotypesare unnecessary or a population with genetic variation is required, and these rats are usually referred to as stocks rather than … The wild-type Sprague Dawley rat thymus was comprised mostly of CD4/CD8 double positive cells whereas in the SRG rat, the viable cells recovered were CD4-/CD8- (Fig 1A–1C). (12) using a protein concentration of 1 mg/ml and a biphenyl concentration of 1.25 mM. Evidence from both clinical research and animal studies has revealed that this syndrome may have fetal origins, with epigenetics being proposed as the underlying mechanism. Rats that underwent adrenalectomy or adrenalectomy and castration were given 0.9% NaCl solution in place of water. Reduced sperm motility and/or increased abnormal sperm were noted in mice, but not in rats, exposed to 3 mg/m 3 or higher in 13-week inhalation studies with cobalt sulfate ( NTP, 2011a ). Navas, in Encyclopedia of Toxicology (Third Edition), 2014. The physical characteristics of the Sprague Dawley rats include two incisors that continuously grow through life which are ‘filed’ down through biting hard material. No histopathological findings were observed in other tissues. For collection of a few selected tissues, young pups at PND 1, 7, and 14 were perfused with NBF via intracardiac instillation of fixative. More than one potential NOAEL can be derived from the results described by Kinkead et al. Almost all laboratory and domestic pet rats belong to a single species, the Norway rat (Rattus norvegicus). The chemical … The aim of this study was to ascertain experimentally, through the model of altering the early mother-infant bond and the late social bond through isolation, the effect on the histologic characteristics of the liver in adult Sprague-Dawley rats subjected to intermittent chronic stress. For example, a NOAEL of 4 mg/kg/day may be protective against TNB-induced encephalitis in female rats (an effect that was evident in mid- and high-dose groups only), while a NOAEL of 23 mg/kg/day may protect against the testicular atrophy that was evident in high-dose males. Sham control groups are treated intranigrally with either 1 μl Ringer's solution or 1.05 μl 0.3 M NaOH. Bilateral adrenalectomy, ovariectomy and orchidectomy were carried out aseptically under anesthesia of Nembutol. Spontaneous Cardiomyopathy in Young Sprague-Dawley Rats: Evaluation of Biological and Environmental Variability FRANCK CHANUT 1,CARIE KIMBROUGH 2,RICK HAILEY 2,BRIAN BERRIDGE 2,ANGELA HUGHES-EARLE 3,RHIANNON DAVIES 1, KIMBERLY ROLAND 3,ALAN STOKES 2,ALESSANDRO CASARTELLI 4,MALCOLM YORK 1,HOLLY JORDAN 2,FEDERICA CRIVELLENTE 4, PATRIZIA … We investigated the effects of chronic administration of MG on glucose tolerance and β-cell insulin secreting mechanism in 12-week-old male Sprague-Dawley rats. 1995a and 1995b). Animals were divided into subgroups to accommodate a number of premating, postmating, and recovery dosing regimens. Its main advantage is its calmness and ease of handling. In order to examine the degree of variability of an outbred stock obtained from various sources, Sprague Dawley (SD) rat lines obtained from two sources were evaluated for their growth characteristics. In addition, competition studies were conducted in order to establish specificity of the radioactivity that concentrates in nuclei of certain cells, by preinjecting 100 x excess of unlabelled hormone of the same or different kind as the subsequently injected labelled hormone. Sprague–Dawley rats were used throughout this study. Background and aims: Daily nutrient intake and growth of male Sprague-Dawley (SD) rats fed compositionally different diets were monitored over 114 weeks to determine whether rats fed ad libitum (AL) or diet restricted (DR) followed normal growth parameters. The objective of this study was to evaluate the toxic characteristics of Rauvolfia tetraphylla leaf extract in Sprague dawley rats after treatment for minimum 7 days by oral route. (Index of major rat strains, with histories and strain characteristics). Dawley ® Rat. The strain was created by R. W. Dawley in 1925, from a hooded male hybrid of unknown origin and an albino female (probably Wistar), and was crossed with the female’s progeny for 7 generations.The SPRAGUE DAWLEY® rat is an albino outbred rat with an elongated head and a … The average litter size of the Sprague Dawley rat is 10.5. Blood was collected by decapitation from 3000 rats between 12.00 and 13.00. Gestation for Sprague Dawley on an average is a cycle of 22 days (Sprague Dawley, 2006). Characteristics of Open Field Behavior of Wistar and Sprague-Dawley Rats Yuzo ASANO Safety Research Laboratory, Tanabe Seiyaku Co., Ltd., Kashima 3-16-89, Yodogawa-ku, Osaka 532, Japan (Received 31 January 1986/Accepted 2 May 1986) The open field test (OFT) was carried out on Wistar and Sprague-Dawley rats The relationship of this change with other causes of endocardial thickening is discussed, and the significance of these alterations is assessed. MATERIALS AND METHODS Four hundred eighty 5-week old Sprague- Dawley, CD, outbred albino rats, 240 males Methods: An automated haematology analyzer was used to determine haematological indices of whole blood from individual in 20 each of male and female inbred Sprague Dawley rats. However, moderate DR did improve survival by delaying the onset and progression of spontaneous degenerative disease and tumors in Sprague-Dawley rats ( Keenan et al. The relationship of this change with other causes of endocardial thickening is discussed, and the significance of these alterations is assessed. We use cookies to help provide and enhance our service and tailor content and ads. The wild-type Sprague Dawley rat thymus was comprised mostly of CD4/CD8 double positive cells whereas in the SRG rat, the viable cells recovered were CD4-/CD8- (Fig 1A–1C). Characteristics of Open Field Behavior of Wistar and Sprague-Dawley Rats Yuzo ASANO Safety Research Laboratory, Tanabe Seiyaku Co., Ltd., Kashima 3-16-89, Yodogawa-ku, Osaka 532, Japan (Received 31 January 1986/Accepted 2 May 1986) The open field test (OFT) was carried out on Wistar and Sprague-Dawley rats between the ages of 3 to 20 weeks. These findings suggest that clues from rats of familiar strains influence helping behavior, whereas clues from rats of unfamiliar strains are largely ignored. F0 female rats were necropsied at termination with pieces of spleen, kidney, and liver processed for histopathological evaluation. However, 2 mg/kg/day would be a LOAEL for the hyaline droplet-related nephropathy that was evident in male rats at all TNB dose levels. Metyrapone binding was studied as decribed previously (9). al., 2000). In contrast to the Sprague- Dawley rats familiar with Long-Evans rats, Sprague-Dawley rats unfamiliar with Long-Evans rats behaved similarly to control rats (Fig. We use cookies to help provide and enhance our service and tailor content and ads. Sprague -Dawley rats are commonly used strains of rats in experimental research due to their metabolic characteristics which are closer to the human and their ability to replicate many symptoms of human conditions (Cortés-Ortiz et al., 2014; Hubert et. Blood was obtained from a peripheral vein of 550 normal human subjects of various ages between 9.00 and 11.00 [6–10, 24]. Methemoglobin was formed in the mid- and high-dose groups for both sexes, although these levels returned to normal in a subset of males allowed to recover after treatment. Decreases in sperm motility and/or increased abnormal sperm were noted in mice, but not in rats, exposed to 3 mg/m3 or more in 13-week inhalation studies with cobalt sulfate (National Toxicology Program, 1991). The doses, as calculated by the authors, were 0, 2, 9, and 19 mg/kg/day for males and 0, 3, 14, and 29 mg/kg/day for females. At necropsy, samples of blood were taken for clinical chemistry/hematological evaluation. Immunohistochemical (IHC) staining was performed on testis and various immune system organs. Ten animals/sex/group received 0, 70, 400, or 800 mg TNB/kg diet, levels calculated by the authors to result in average doses of 0, 3, 23, and 51 mg/kg/day in males and 0, 4, 30, and 60 mg/kg/day in females (0, 8, 55, and 110 mg/kg/day during lactation). MATERIALS AND METHODS Four hundred eighty 5-week old Sprague- Dawley, CD, outbred albino rats, 240 males The NOEL was set at 25 μg kg−1 per day, and the LOEL was set at 125 μg kg−1 per day, based on spongy degeneration observed in nervous tissue. Determinations were in all cases made in triplicate on progressive aliquots. Slides were counterstained with hematoxylin followed by a bluing agent, and then rinsed with reaction buffer prior to dehydration and coverslipping. The Sprague-Dawley rat is a hybrid albino strain with a long narrow head. Neurological signs, for example, head tilt and loss of equilibrium, were observed in lactating dams. A total of 24 male rats were randomly allocated to three treatments and administered either saline or 35 mg Zn/kg body weight from zinc sulphate (ZnSO 4 ) or Zn–Gly by feeding tube separately. In this single-dose study, the metabonomic profile changes in the 48 h after orally administrated 3-MCPD dipalmitate at 1600 mg/kg BW to Sprague–Dawley (SD) rats were determined with liquid chromatography (LC) coupled with mass spectrometry (MS) system. The current study aims to investigate the influence of five rare earth elements (REEs) (i.e., lanthanum (La), cerium (Ce), praseodymium (Pr), neodymium (Nd), and gadolinium (Gd)) on the growth of Sprague–Dawley (SD) rats, and to explore the accumulation characteristics of REEs in tissues and organs with different doses as well as the detoxification and elimination of high-dose REEs. injection of pentylenetetrazol (PTZ, 70–80 mg kg−1); some pups of each litter were used as controls. The strain was created by R. W. Dawley in 1925, from a hooded male hybrid of unknown origin and an albino female (probably Wistar), and was crossed with the female’s progeny for 7 generations.The SPRAGUE DAWLEY® rat is an albino outbred rat with an elongated head … The state of the estrus cycle was determined and was found to have no influence on the parameters tested. The images presented in the Atlas were extracted from the Nanozoomer scans by use of the NDP.View II software from Hamamatsu Corp. Histologic sections were scanned with a Hamamatsu Nanozoomer slide scanner, most commonly using the 40x scan objective. Activity evaluation tests, though with no evidence of compound-related depletions in motor skills methods! The Sprague Dawley, Inc. NIH received stock from Sprague Dawley rat is 10.5 min−1 microinfusion! The methods described by Creaven et al obtained from various sources, Sprague Dawley, )! Has a wide array of sprague dawley rats characteristics research use including in the drinking water 125... 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Major characteristics of polycystic ovary syndrome ( PCOS ) the Fourth International Congress on Hormonal Steroids, 1979 a! Left thoracotomy was performed through the Fourth intercostal space using a sterile technique was on. All groups at termination with pieces of spleen, kidney, and throughout.